Identification of new eligible indicator organisms for combined sewer overflow via 16S rRNA gene amplicon sequencing in Kanda River, Tokyo

Daniel Ekhlas, Futoshi Kurisu*, Ikuro Kasuga, Tomislav Cernava, Gabriele Berg, Miaomiao Liu, Hiroaki Furumai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Fecal indicator bacteria (FIB) are commonly used to evaluate the pollution impact of combined sewer overflows (CSOs) in urban rivers. Although water quality assessment with FIB has a long tradition, recent studies demonstrated that FIB have a low correlation with pathogens and therefore are not accurate enough for the assessment of potential human hazards in water. Consequently, new eligible and more specific indicators have to be identified, which was done in this study via sequencing of genetic markers from total community DNA. To identify potential microbiome-based indicators, microbial communities in samples from an urban river in Tokyo under different climatic conditions (dry and rainy) were compared with the influent and effluent of three domestic wastewater treatment plants (WWTPs) by analyzing 16 S rRNA gene amplicon libraries. In the first part of this study, physicochemical parameters and FIB …
Original languageEnglish
Article number112059
JournalJournal of Environmental Management
Publication statusPublished - 15 Apr 2021


  • Combined sewer overflow
  • Fecal indicator bacteria
  • Fecal pollution
  • Microbiome analysis

ASJC Scopus subject areas

  • Waste Management and Disposal
  • Management, Monitoring, Policy and Law
  • Environmental Engineering

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