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Abstract
Fatty aldehyde production by chemical synthesis causes an immense burden to the environment. Within in this study, we explored a sustainable, aldehyde‐selective and mild alternative approach by utilizing carboxylic acid reductases (CARs). CARs from Neurospora crassa ( Nc CAR), Thermothelomyces thermophila ( Tt CAR), Nocardia iowensis ( Ni CAR), Mycobacterium marinum ( MmC AR) and Trametes versicolor ( Tv CAR) were overexpressed in E. coli K‐12 MG1655 RARE (DE3) and screened for medium‐ to long‐chain fatty acid (C6‐C18) reduction. MmC AR showed the broadest tolerance towards all carbon‐chain lengths and was selected for further investigations of fatty aldehyde synthesis in whole cells. To yield relevant product concentrations, different limitations of CAR whole cell conversions were elucidated and compensated. We coupled an in vitro cofactor recycling system to a whole cell biocatalyst to support cofactor supply and achieved 12.36 g L ‐ 1 of octanal (STY 0.458 g L ‐1 h ‐1 ) with less than 1.5 % of 1‐octanol.
Original language | English |
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Pages (from-to) | 5076-5090 |
Number of pages | 15 |
Journal | ChemCatChem |
Volume | 12 |
Issue number | 20 |
DOIs | |
Publication status | Published - 20 Oct 2020 |
Keywords
- aldehydes
- carboxylic acid reductase
- enzymatic reduction in vivo
- fatty acids
- green chemistry
ASJC Scopus subject areas
- Catalysis
- Inorganic Chemistry
- Physical and Theoretical Chemistry
- Organic Chemistry
Fields of Expertise
- Human- & Biotechnology
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- 1 Poster presentation
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WHOLE CELL PRODUCTION OF FATTY ALDEHYDES BY MYCOBACTERIUM MARINUM CARBOXYLATE REDUCTASE
Melissa Horvat (Speaker) & Margit Winkler (Contributor)
19 Jul 2021 → 22 Jul 2021Activity: Talk or presentation › Poster presentation › Science to science