Influence of the Synthesis and Storage Conditions on the Activity of Candida antarctica Lipase B ZIF-8 Biocomposites

Natasha K. Maddigan, Oliver M. Linder-Patton, Paolo Falcaro, Christopher J. Sumby, Stephen G. Bell, Christian J. Doonan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The biomimetic mineralization of zeolitic imidazolate framework-8 (ZIF-8) has been reported as a strategy for enzyme immobilization, enabling the heterogenization and protection of biomacromolecules. Here, we report the preparation of different Candida antarctica lipase B biocomposites (CALB@ZIF-8) formed by altering the concentrations of Zn2+ and 2-methylimidazole (2-mIM). The influence of synthetic conditions on the catalytic activity of the lipase CALB was examined by hydrolysis and transesterification assays in aqueous and organic media, respectively. We demonstrated that for both reactions, activity was retained for the biocomposites formed at low Zn2+/2-mIM ratios but notably almost entirely lost when the ligand concentration used to form the biocomposites was increased. Additionally, phosphate buffer could regenerate the activity of larger particles by degrading the crystal surfaces and releasing encapsulated CALB into solution. Transesterification reactions using CALB@ZIF-8 biocomposites were undertaken in 100% hexane, giving rise to enhanced CALB activity relative to the free enzyme. These observations highlight the fundamental importance of synthetic protocols and operating parameters for developing enzyme@MOF biocomposites with improved activity in challenging conditions.

Original languageEnglish
Pages (from-to)51867-51875
Number of pages9
JournalACS Applied Materials and Interfaces
Issue number44
Publication statusPublished - 10 Nov 2021


  • biomimetic mineralization
  • Candida antarctica lipase B
  • enzyme biocomposites
  • hydrolysis reactions
  • transesterification reactions
  • ZIF-8 biocomposites

ASJC Scopus subject areas

  • Materials Science(all)


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