Mining for nonribosomal peptide synthetase and polyketide synthase genes revealed a high level of diversity in the Sphagnum bog metagenome

Christina Andrea Müller, Lisa Oberauner-Wappis, Armin Peymann, Gregory C.A. Amos, Elizabeth M. H. Wellington, Gabriele Berg

Research output: Contribution to journalArticlepeer-review


Sphagnum bog ecosystems are among the oldest vegetation forms harboring a specific microbial community and are known to produce an exceptionally wide variety of bioactive substances. Although the Sphagnum metagenome shows a rich secondary metabolism, the genes have not yet been explored. To analyze nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs), the diversity of NRPS and PKS genes in Sphagnum-associated metagenomes was investigated by in silico data mining and sequence-based screening (PCR amplification of 9,500 fosmid clones). The in silico Illumina-based metagenomic approach resulted in the identification of 279 NRPSs and 346 PKSs, as well as 40 PKS-NRPS hybrid gene sequences. The occurrence of NRPS sequences was strongly dominated by the members of the Protebacteria phylum, especially by species of the Burkholderia genus, while PKS …
Original languageEnglish
Pages (from-to)5064
Number of pages5072
JournalApplied and Environmental Microbiology
Publication statusPublished - 22 May 2015

Fields of Expertise

  • Human- & Biotechnology

Treatment code (Nähere Zuordnung)

  • Experimental

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