Molecular cloning, expression, and characterization of acyltransferase from Pseudomonas protegens

Nina G. Schmidt, Anna Żądło-Dobrowolska, Valerie Ruppert, Christian Höflehner, Birgit Wiltschi, Wolfgang Kroutil*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The formation of C-C bonds by using CoA independent acyltransferases may have significant impact for novel methods for biotechnology. We report the identification of Pseudomonas strains with CoA-independent acyltransferase activity as well as the heterologous expression of the enzyme in E. coli. The cloning strategies and selected expression studies are discussed. The recombinant acyltransferases were characterized with regard to thermal and storage stability, pH,- and co-solvent tolerance. Moreover, the impact of bivalent metals, inhibitors, and other additives was tested. Careful selection of expression and working conditions led to obtain recombinant acyltransferase form Pseudomonas protegens with up to 11 U mL −1 activity.

Original languageEnglish
Pages (from-to)6057-6068
Number of pages12
JournalApplied Microbiology and Biotechnology
Issue number14
Publication statusPublished - 12 May 2018


  • 2,4-diacetylphloroglucinol
  • Acyltransferase
  • Friedel-Crafts reaction
  • Pseudomonas protegens

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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