Nitrile hydrolysis activity of Rhodococcus erythropolis NCIMB 11540 whole cells.

Mandy K.S. Vink, R Wijtmans, C Reisinger, Rutger J.F. Van den Berg, Christien A. Schortinghuis, Helmut Schwab, Hans E. Schoemaker, Floris P.J.T. Rutjes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The nitrile hydrolyzing properties of the bacterium strain Rhodococcus erythropolis NCIMB 11540 have been investigated. Using whole cells of the microorganism, a wide variety of aromatic and aliphatic cyanide-containing substrates was successfully hydrolyzed to the corresponding amide or acid. In the case of dicyanides, selective monohydrolysis took place, which was further explored in the desymmetrization of malononitriles resulting in the corresponding cyano amides in enantiomeric excesses of up to 98%.
Original languageEnglish
Pages (from-to)569-573
JournalJournal of Biotechnology
Issue number5
Publication statusPublished - 2006

Treatment code (Nähere Zuordnung)

  • Basic - Fundamental (Grundlagenforschung)

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