Optical Ammonia Sensor for Continuous Bioprocess Monitoring

Maximilian Maierhofer; Sergey Borisov; Torsten Mayr

Optical Ammonia Sensor for Continuous Bioprocess Monitoring

Maximilian Maierhofer, Sergey Borisov, Torsten Mayr

Institute of Analytical Chemistry and Food Chemistry (6450)

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › peer-review

Abstract

We present an optical ammonia sensor suitable for bioprocess monitoring. A fluorescent dye is physically entrapped in a polyurethane hydrogel forming an emulsion system with vinylterminated polydimethylsiloxane (PDMS). The sensing layer is covered by a hydrophobic porous membrane which excludes hydrophilic substances. Ammonia, diffuses through this barrier and PDMS to the protonated dye, whereby it deprotonates the dye and switches off its emission. Readout is performed with a miniaturized phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) acts as detection method and Egyptian blue as reference material.

Original language	English
Title of host publication	Proceedings Eurosensors 2018
Editors	Anton Köck, Marco Deluca
Publisher	MDPI AG
Number of pages	4
Volume	2
Publication status	Published - 19 Nov 2018
Event	Eurosensors 2018 - Karl Franzens Universität Graz, Graz, Austria Duration: 9 Sept 2018 → 12 Sept 2018 https://www.eurosensors2018.eu

Conference

Conference	Eurosensors 2018
Country/Territory	Austria
City	Graz
Period	9/09/18 → 12/09/18
Internet address	https://www.eurosensors2018.eu

Keywords

Ammonia
optical sensor
process monitoring
fluorescent dye

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https://www.mdpi.com/2504-3900/2/13/1041

Cite this

@inproceedings{1509ae404cd54a98a0679b563add6f38,

title = "Optical Ammonia Sensor for Continuous Bioprocess Monitoring",

abstract = "We present an optical ammonia sensor suitable for bioprocess monitoring. A fluorescent dye is physically entrapped in a polyurethane hydrogel forming an emulsion system with vinylterminated polydimethylsiloxane (PDMS). The sensing layer is covered by a hydrophobic porous membrane which excludes hydrophilic substances. Ammonia, diffuses through this barrier and PDMS to the protonated dye, whereby it deprotonates the dye and switches off its emission. Readout is performed with a miniaturized phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) acts as detection method and Egyptian blue as reference material. ",

keywords = "Optischer Sensor, Ammoniak, Bioprozess{\"u}berwachung, Fluoreszenz, Ammonia, optical sensor, process monitoring, fluorescent dye",

author = "Maximilian Maierhofer and Sergey Borisov and Torsten Mayr",

year = "2018",

month = nov,

day = "19",

language = "English",

volume = "2",

editor = "Anton K{\"o}ck and Marco Deluca",

booktitle = "Proceedings Eurosensors 2018",

publisher = "MDPI AG",

address = "Switzerland",

note = "Eurosensors 2018 ; Conference date: 09-09-2018 Through 12-09-2018",

url = "https://www.eurosensors2018.eu",

}

TY - GEN

T1 - Optical Ammonia Sensor for Continuous Bioprocess Monitoring

AU - Maierhofer, Maximilian

AU - Borisov, Sergey

AU - Mayr, Torsten

PY - 2018/11/19

Y1 - 2018/11/19

N2 - We present an optical ammonia sensor suitable for bioprocess monitoring. A fluorescent dye is physically entrapped in a polyurethane hydrogel forming an emulsion system with vinylterminated polydimethylsiloxane (PDMS). The sensing layer is covered by a hydrophobic porous membrane which excludes hydrophilic substances. Ammonia, diffuses through this barrier and PDMS to the protonated dye, whereby it deprotonates the dye and switches off its emission. Readout is performed with a miniaturized phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) acts as detection method and Egyptian blue as reference material.

AB - We present an optical ammonia sensor suitable for bioprocess monitoring. A fluorescent dye is physically entrapped in a polyurethane hydrogel forming an emulsion system with vinylterminated polydimethylsiloxane (PDMS). The sensing layer is covered by a hydrophobic porous membrane which excludes hydrophilic substances. Ammonia, diffuses through this barrier and PDMS to the protonated dye, whereby it deprotonates the dye and switches off its emission. Readout is performed with a miniaturized phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) acts as detection method and Egyptian blue as reference material.

KW - Optischer Sensor

KW - Ammoniak

KW - Bioprozessüberwachung

KW - Fluoreszenz

KW - Ammonia

KW - optical sensor

KW - process monitoring

KW - fluorescent dye

M3 - Conference paper

VL - 2

BT - Proceedings Eurosensors 2018

A2 - Köck, Anton

A2 - Deluca, Marco

PB - MDPI AG

T2 - Eurosensors 2018

Y2 - 9 September 2018 through 12 September 2018

ER -

Optical Ammonia Sensor for Continuous Bioprocess Monitoring

Abstract

Conference

Keywords

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