TY - JOUR
T1 - Polysaccharide thin solid films for analgesic drug delivery and growth of human skin cells
AU - Maver, Tina
AU - Mohan, Tamilselvan
AU - Gradišnik, Lidija
AU - Finšgar, Matjaž
AU - Stana Kleinschek, Karin
AU - Maver, Uroš
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Chronic wounds not only lower the quality of patient's life significantly, but also present a huge financial burden for the healthcare systems around the world. Treatment of larger wounds often requires the use of more complex materials, which can ensure a successful renewal or replacement of damaged or destroyed tissues. Despite a range of advanced wound dressings that can facilitate wound healing, there are still no clinically used dressings for effective local pain management. Herein, alginate (ALG) and carboxymethyl cellulose (CMC), two of the most commonly used materials in the field of chronic wound care, and combination of ALG-CMC were used to create a model wound dressing system in the form of multi-layered thin solid films using the spin-assisted layer-by-layer (LBL) coating technique. The latter multi-layer system was used to incorporate and study the release kinetics of analgesic drugs such as diclofenac and lidocaine at physiological conditions. The wettability, morphology, physicochemical and surface properties of the coated films were evaluated using different surface sensitive analytical tools. The influence of in situ incorporated drug molecules on the surface properties (e.g., roughness) and on the proliferation of human skin cells (keratinocytes and skin fibroblasts) was further evaluated. The results obtained from this preliminary study should be considered as the basis for the development "real" wound dressing materials and for 3D bio-printing applications.
AB - Chronic wounds not only lower the quality of patient's life significantly, but also present a huge financial burden for the healthcare systems around the world. Treatment of larger wounds often requires the use of more complex materials, which can ensure a successful renewal or replacement of damaged or destroyed tissues. Despite a range of advanced wound dressings that can facilitate wound healing, there are still no clinically used dressings for effective local pain management. Herein, alginate (ALG) and carboxymethyl cellulose (CMC), two of the most commonly used materials in the field of chronic wound care, and combination of ALG-CMC were used to create a model wound dressing system in the form of multi-layered thin solid films using the spin-assisted layer-by-layer (LBL) coating technique. The latter multi-layer system was used to incorporate and study the release kinetics of analgesic drugs such as diclofenac and lidocaine at physiological conditions. The wettability, morphology, physicochemical and surface properties of the coated films were evaluated using different surface sensitive analytical tools. The influence of in situ incorporated drug molecules on the surface properties (e.g., roughness) and on the proliferation of human skin cells (keratinocytes and skin fibroblasts) was further evaluated. The results obtained from this preliminary study should be considered as the basis for the development "real" wound dressing materials and for 3D bio-printing applications.
KW - Alginate
KW - Carboxymethyl cellulose
KW - Diclofenac
KW - Keratinocytes
KW - Lidocaine
KW - Skin fibroblasts
KW - Thin (blend) films
UR - http://www.scopus.com/inward/record.url?scp=85064632910&partnerID=8YFLogxK
U2 - 10.3389/fchem.2019.00217
DO - 10.3389/fchem.2019.00217
M3 - Article
AN - SCOPUS:85064632910
SN - 2296-2646
VL - 7
JO - Frontiers in Chemistry
JF - Frontiers in Chemistry
IS - APR
M1 - 217
ER -