TY - JOUR
T1 - Quo vadis? Microbial profiling revealed strong effects of cleanroom maintenance and routes of contamination in indoor environments
AU - Moissl-Eichinger, Christine
AU - Auerbach, Anna K.
AU - Probst, Alexander J.
AU - Mahnert, Alexander
AU - Lauren, Tom
AU - Piceno, Yvette
AU - Andersen, Gary L.
AU - Venkateswaran, Kasthuri
AU - Rettberg, Petra
AU - Barczyk, Simon
AU - Pukall, Rüdiger
AU - Berg, Gabriele
PY - 2015
Y1 - 2015
N2 - Space agencies maintain highly controlled cleanrooms to ensure the demands of planetary protection. To study potential effects of microbiome control, we analyzed microbial communities in two particulate-controlled cleanrooms (ISO 5 and ISO 8) and two vicinal uncontrolled areas (office, changing room) by cultivation and 16S rRNA gene amplicon analysis (cloning, pyrotagsequencing and PhyloChip G3 analysis). Maintenance procedures affected the microbiome on total abundance and microbial community structure concerning richness, diversity and relative abundance of certain taxa. Cleanroom areas were found to be mainly predominated by potentially human-associated bacteria; archaeal signatures were detected in every area. Results indicate that microorganisms were mainly spread from the changing room (68%) into the cleanrooms, potentially carried along with human activity. The numbers of colony …
AB - Space agencies maintain highly controlled cleanrooms to ensure the demands of planetary protection. To study potential effects of microbiome control, we analyzed microbial communities in two particulate-controlled cleanrooms (ISO 5 and ISO 8) and two vicinal uncontrolled areas (office, changing room) by cultivation and 16S rRNA gene amplicon analysis (cloning, pyrotagsequencing and PhyloChip G3 analysis). Maintenance procedures affected the microbiome on total abundance and microbial community structure concerning richness, diversity and relative abundance of certain taxa. Cleanroom areas were found to be mainly predominated by potentially human-associated bacteria; archaeal signatures were detected in every area. Results indicate that microorganisms were mainly spread from the changing room (68%) into the cleanrooms, potentially carried along with human activity. The numbers of colony …
UR - http://www.nature.com/srep/
UR - http://www.nature.com/articles/srep09156
U2 - 10.1038/srep09156
DO - 10.1038/srep09156
M3 - Article
VL - 5
SP - 1
EP - 13
JO - Scientific Reports
JF - Scientific Reports
IS - 9156
ER -