Synthetic Core Promoters for Pichia pastoris

Thomas Vogl, Claudia Ruth, Julia Pitzer, Thomas Kickenweiz, Anton Glieder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Synthetic promoters are commonly used tools for circuit design or high level protein production. Promoter engineering efforts in yeasts, such as Saccharomyces cerevisiae and Pichia pastoris have mostly been focused on altering upstream regulatory sequences such as transcription factor binding sites. In higher eukaryotes synthetic core promoters, directly needed for transcription initiation by RNA Polymerase II, have been successfully designed. Here we report the first synthetic yeast core promoter for P. pastoris, based on natural yeast core promoters. Furthermore we used this synthetic core promoter sequence to engineer the core promoter of the natural AOX1 promoter, thereby creating a set of core promoters providing a range of different expression levels. As opposed to engineering strategies of the significantly longer entire promoter, such short core promoters can directly be added on a PCR primer facilitating library generation and are sufficient to obtain variable expression yields.
Original languageEnglish
Pages (from-to)188–191
JournalACS Synthetic Biology
Issue number3
Publication statusPublished - 2014

Fields of Expertise

  • Human- & Biotechnology

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