Utilization of desugarized sugar beet molasses for the production of poly(3-hydroxybutyrate) by halophilic Bacillus megaterium uyuni S29

Maximilian T. Schmid, Hyunjeong Song, Michaela Raschbauer, Florian Emerstorfer, Markus Omann, Franz Stelzer, Markus Neureiter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Desugarized sugar beet molasses is a high-salinity side stream after fractionation of beet molasses by chromatography. It could be demonstrated that this by-product of the sugar industry is a suitable substrate for the production of poly(3-hydroxybutyrate) with the halophilic bacterium Bacillus megaterium uyuni S29. Cell dry mass concentrations of up to 16.7 g L−1 with a P(3HB) content of 0.6 g g−1 were achieved after only 24 h of batch cultivation. Utilization of a desugarized sugar beet molasses medium resulted in a two- to threefold increase in biomass production compared to a conventional mineral based medium. Depending on the extraction method, the molecular mass (Mn) of the P(3HB) varied between 119 kDa and 722 kDa. The high P(3HB) productivity of 0.42 g L−1 h−1 during batch experiments combined with desugarized sugar beet molasses as inexpensive substrate suggests a simple and robust process for cost effective P(3HB) production.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalProcess Biochemistry
Publication statusPublished - 1 Nov 2019


  • Bacillus megaterium
  • Desugarized sugar beet molasses
  • Halophilic fermentation
  • Poly(3-hydroxybutyrate)
  • Sugar industry by-product

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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