Abstract
Desugarized sugar beet molasses is a high-salinity side stream after fractionation of beet molasses by chromatography. It could be demonstrated that this by-product of the sugar industry is a suitable substrate for the production of poly(3-hydroxybutyrate) with the halophilic bacterium Bacillus megaterium uyuni S29. Cell dry mass concentrations of up to 16.7 g L−1 with a P(3HB) content of 0.6 g g−1 were achieved after only 24 h of batch cultivation. Utilization of a desugarized sugar beet molasses medium resulted in a two- to threefold increase in biomass production compared to a conventional mineral based medium. Depending on the extraction method, the molecular mass (Mn) of the P(3HB) varied between 119 kDa and 722 kDa. The high P(3HB) productivity of 0.42 g L−1 h−1 during batch experiments combined with desugarized sugar beet molasses as inexpensive substrate suggests a simple and robust process for cost effective P(3HB) production.
Original language | English |
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Pages (from-to) | 9-15 |
Number of pages | 7 |
Journal | Process Biochemistry |
Volume | 86 |
DOIs | |
Publication status | Published - 1 Nov 2019 |
Keywords
- Bacillus megaterium
- Desugarized sugar beet molasses
- Halophilic fermentation
- Poly(3-hydroxybutyrate)
- Sugar industry by-product
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology