TY - JOUR
T1 - Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii
AU - Barone, Giovanni Davide
AU - Emmerstorfer-Augustin, Anita
AU - Biundo, Antonino
AU - Pisano, Isabella
AU - Coccetti, Paola
AU - Mapelli, Valeria
AU - Camattari, Andrea
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/3
Y1 - 2023/3
N2 - Since the mid-1960s, methylotrophic yeast Komagataella phaffii (previously described as Pichia pastoris) has received increasing scientific attention. The interest for the industrial production of proteins for different applications (e.g., feed, food additives, detergent, waste treatment processes, and textile) is a well-consolidated scientific topic, and the importance for this approach is rising in the current era of environmental transition in human societies. This review aims to summarize fundamental and specific information in this scientific field. Additionally, an updated description of the relevant products produced with K. phaffii at industrial levels by a variety of companies—describing how the industry has leveraged its key features, from products for the ingredients of meat-free burgers (e.g., IMPOSSIBLE™ FOODS, USA) to diabetes therapeutics (e.g., Biocon, India)—is provided. Furthermore, active patents and the typical workflow for industrial protein production with this strain are reported.
AB - Since the mid-1960s, methylotrophic yeast Komagataella phaffii (previously described as Pichia pastoris) has received increasing scientific attention. The interest for the industrial production of proteins for different applications (e.g., feed, food additives, detergent, waste treatment processes, and textile) is a well-consolidated scientific topic, and the importance for this approach is rising in the current era of environmental transition in human societies. This review aims to summarize fundamental and specific information in this scientific field. Additionally, an updated description of the relevant products produced with K. phaffii at industrial levels by a variety of companies—describing how the industry has leveraged its key features, from products for the ingredients of meat-free burgers (e.g., IMPOSSIBLE™ FOODS, USA) to diabetes therapeutics (e.g., Biocon, India)—is provided. Furthermore, active patents and the typical workflow for industrial protein production with this strain are reported.
KW - applied biotechnology
KW - bioreactor-based approaches
KW - biotechnology
KW - industrial biotechnology
KW - Komagataella phaffii
KW - methylotrophic yeast
KW - Pichia pastoris
KW - protein production
UR - http://www.scopus.com/inward/record.url?scp=85151107726&partnerID=8YFLogxK
U2 - 10.3390/biom13030441
DO - 10.3390/biom13030441
M3 - Review article
C2 - 36979376
AN - SCOPUS:85151107726
SN - 2218-273X
VL - 13
JO - Biomolecules
JF - Biomolecules
IS - 3
M1 - 441
ER -