LOVTRAP: an optogenetic system for photoinduced protein dissociation

Hui Wang; Marco Vilela; Andreas Winkler; Miroslaw Tarnawski; Ilme Schlichting; Hayretin Yumerefendi; Brian Kuhlman; Rihe Liu; Gaudenz Danuser; Klaus M Hahn

doi:10.1038/nmeth.3926

LOVTRAP: an optogenetic system for photoinduced protein dissociation

Hui Wang, Marco Vilela, Andreas Winkler, Miroslaw Tarnawski, Ilme Schlichting, Hayretin Yumerefendi, Brian Kuhlman, Rihe Liu, Gaudenz Danuser, Klaus M Hahn

Institut für Biochemie (6480)

Publikation: Beitrag in einer Fachzeitschrift › Artikel › Begutachtung

Abstract

LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

Originalsprache	englisch
Seiten (von - bis)	755-758
Fachzeitschrift	Nature Methods
Jahrgang	13
DOIs	https://doi.org/10.1038/nmeth.3926
Publikationsstatus	Veröffentlicht - 2016

ASJC Scopus subject areas

Biochemie, Genetik und Molekularbiologie (sonstige)

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10.1038/nmeth.3926

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abstract = "LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.",

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AU - Wang, Hui

AU - Vilela, Marco

AU - Winkler, Andreas

AU - Tarnawski, Miroslaw

AU - Schlichting, Ilme

AU - Yumerefendi, Hayretin

AU - Kuhlman, Brian

AU - Liu, Rihe

AU - Danuser, Gaudenz

AU - Hahn, Klaus M

PY - 2016

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N2 - LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

AB - LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

KW - optogenetics

KW - cell signalling

KW - cell migration

U2 - 10.1038/nmeth.3926

DO - 10.1038/nmeth.3926

M3 - Article

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VL - 13

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JO - Nature Methods

JF - Nature Methods

ER -

LOVTRAP: an optogenetic system for photoinduced protein dissociation

Abstract

ASJC Scopus subject areas

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