TY - JOUR
T1 - Temperature-induced modulation of the electrophysiological behavior of A549 lung cancer cells
AU - Langthaler, Sonja
AU - Rienmüller, Theresa Margarethe
AU - Lozanovic Sajic, Jasmina
AU - Baumgartner, Christian
PY - 2022/8/1
Y1 - 2022/8/1
N2 - Cancer cells typically exhibit altered ion channel expression and membrane potential, which plays an important role in various cellular processes such as proliferation or apoptosis. Hence, therapeutic strategies that modulate ion channel activity or shift the membrane potential prove to be promising for cancer therapy. Since temperature significantly affects ion channel function, deviations from normal body temperature might provide a key factor in electrophysiological processes, potentially affecting cell proliferation and thus tumor progression. In this pilot study, we focused on a first insight into the temperature-induced modulation of A549 cells using an automated patch clamp system. Measurements of 14 cells at hypo-, normo- and hyperthermia were accomplished to investigate the temperature-dependent electrophysiological behavior. The results revealed that both, temperatures below and above normal body temperature affect the current and potential of A549 cells. In hyperthermia the potential tends to more hyperpolarized values, while conversely, in hypothermic conditions we observed mainly a lower current response and thus a depolarization of the membrane potential.
AB - Cancer cells typically exhibit altered ion channel expression and membrane potential, which plays an important role in various cellular processes such as proliferation or apoptosis. Hence, therapeutic strategies that modulate ion channel activity or shift the membrane potential prove to be promising for cancer therapy. Since temperature significantly affects ion channel function, deviations from normal body temperature might provide a key factor in electrophysiological processes, potentially affecting cell proliferation and thus tumor progression. In this pilot study, we focused on a first insight into the temperature-induced modulation of A549 cells using an automated patch clamp system. Measurements of 14 cells at hypo-, normo- and hyperthermia were accomplished to investigate the temperature-dependent electrophysiological behavior. The results revealed that both, temperatures below and above normal body temperature affect the current and potential of A549 cells. In hyperthermia the potential tends to more hyperpolarized values, while conversely, in hypothermic conditions we observed mainly a lower current response and thus a depolarization of the membrane potential.
KW - ion channel kinetics
KW - membrane potential
KW - oncological hyperthermia
KW - temperature modulation
UR - http://www.scopus.com/inward/record.url?scp=85137717695&partnerID=8YFLogxK
U2 - 10.1515/cdbme-2022-1023
DO - 10.1515/cdbme-2022-1023
M3 - Article
SN - 2364-5504
VL - 8
SP - 85
EP - 88
JO - Current Directions in Biomedical Engineering
JF - Current Directions in Biomedical Engineering
IS - 2
ER -