Abstract
Some aromatic amines (AA) have been classified as carcinogens to humans. After entering the body, mainly through tobacco smoke, they can be detected in urine. Thus, their trace analysis as biomarkers in biofluids is of high relevance and can be achieved with gas chromatography (GC–MS), usually after derivatization. This study compares three gas chromatographic methods for the analysis of ten iodinated derivatives of AA: GC–MS in single-ion monitoring (SIM) mode with (1) electron ionization (GC-EI-MS) and (2) negative chemical ionization (GC-NCI-MS), and (3) GC-EI-MS/MS in multiple reaction monitoring (MRM) mode using electron ionization. All methods and most analytes showed good coefficients of determination (R2 > 0.99) for broad linear ranges covering three to five orders of magnitude in the picogram-per-liter to nanogram-per-liter range, with one and two exceptions for (1) and (2) respectively. Excellent limits of detection (LODs) of 9–50, 3.0–7.3, and 0.9–3.9 pg/L were observed for (1), (2), and (3) respectively, and good precision was achieved (intra-day repeatability
Original language | English |
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Pages (from-to) | 3313–3325 |
Number of pages | 13 |
Journal | Analytical and Bioanalytical Chemistry |
Volume | 415 |
Issue number | 17 |
DOIs | |
Publication status | Published - Jul 2023 |
Keywords
- Aromatic amines
- Derivatization
- Gas chromatography-mass spectrometry (GC–MS)
- Gas chromatography-tandem mass spectrometry (GC–MS/MS)
- Negative chemical ionization (NCI)
- Urine
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry