Effects of oxidized phospholipids on gene expression in RAW 264.7 macrophages: a microarray study.

Daniel Koller, Hubert Hackl, Juliane Gertrude Bogner-Strauß, Albin Hermetter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Oxidized phospholipids (oxPLs) are components of oxidized LDL (oxLDL). It is known that oxLDL activates expression of a series of atherogenic genes and their oxPLs contribute to their biological activities. In this study we present the effects of 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine (PGPC) and 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine (POVPC) on gene expression in RAW 264.7 macrophages using cDNA microarrays. PGPC affected the regulation of 146 genes, whereas POVPC showed only very minor effects. PGPC preferentially influenced expression of genes related to cell death, angiogenesis, cholesterol efflux, procoagulant mechanisms, atherogenesis, inflammation, and cell cycle. Many of these effects are known from studies with oxLDL or oxidized 1-hexadecanoyl-2-eicosatetra-5′,8′,11′,14′-enoyl-sn-glycero-3-phosphocholine (oxPAPC), containing PGPC in addition to other oxPL species. It is known that POVPC efficiently reacts with proteins by Schiff base formation, whereas PGPC only physically interacts with its biological targets. POVPC seems to affect cell physiology to a great extent on the protein level, whereas PGPC gives rise to both the modulation of protein function and regulation on the transcriptional level.
Original languageEnglish
Article numbere110486
Number of pages11
JournalPLoS ONE
Issue number10
Publication statusPublished - 2014

Fields of Expertise

  • Human- & Biotechnology


Dive into the research topics of 'Effects of oxidized phospholipids on gene expression in RAW 264.7 macrophages: a microarray study.'. Together they form a unique fingerprint.

Cite this