Projects per year
Abstract
Regulatory proteins play a crucial role in adaptation to environmental cues. Especially for lifestyle transitions, such as cell proliferation or apoptosis, switch-like characteristics are desirable. While nature frequently uses regulatory circuits to amplify or dampen signals, stand-alone protein switches are interesting for applications like biosensors, diagnostic tools, or optogenetics. However, such stand-alone systems frequently feature limited dynamic and operational ranges and suffer from slow response times. Here, we characterize a LOV-activated diguanylate cyclase (LadC) that offers precise temporal and spatial control of enzymatic activity with an exceptionally high dynamic range over four orders of magnitude. To establish this pronounced activation, the enzyme exhibits a two-stage activation process in which its activity is inhibited in the dark by caging its effector domains and stimulated upon illumination by the formation of an extended coiled-coil. These switch-like characteristics of the LadC system can be used to develop new optogenetic tools with tight regulation.
Original language | English |
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Article number | eadh4721 |
Journal | Science Advances |
Volume | 9 |
Issue number | 31 |
DOIs | |
Publication status | Published - 4 Aug 2023 |
Keywords
- photoreceptor
- blue light
- diguanylate cyclase
- LOV
- GGDEF
- switch-like
ASJC Scopus subject areas
- General
Fields of Expertise
- Human- & Biotechnology
Fingerprint
Dive into the research topics of 'Illuminating the inner workings of a natural protein switch: Blue-light sensing in LOV-activated diguanylate cyclases'. Together they form a unique fingerprint.Projects
- 1 Active
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FWF - LOV-DGC - Molecular mechanisms of LOV-regulated diguanylate cyclases
1/10/21 → 30/09/25
Project: Research project
Activities
- 1 Talk at conference or symposium
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Naturally occurring ON-OFF switches: Blue light-regulated LOV-diguanylate cyclases
Ursula Vide (Speaker) & Andreas Winkler (Contributor)
31 Aug 2023Activity: Talk or presentation › Talk at conference or symposium › Science to science